Fly artifact documentation of chrysomya megacephala. A series of experiments were conducted to investigate the effect of fat content in the diet on larval growth in chrysomya megacephala fabricius diptera. Larval competition of chrysomya megacephala and chrysomya rufifacies diptera. Blowflies can locate an odor source with great spatial precision and deposit. The insecticidal effects of this plant were also measured on the first and second instar larvae of anticarsia gemmatalis and spodoptera frugiperda. Thus they mechanically transmit human and animal pathogens ie, viruses, bacteria, protozoan cyst and helminth eggs, by. The occurrence of dipteran fly, chrysomya megacephala fabricius, 1794 is. Chrysomya megacephala is a blow fly species of medical and forensic importance worldwide. Larval competition of chrysomya megacephala and chrysomya. Chrysomya megacephala is a forensically important blow fly distributed in many parts of the world and available throughout the year in northwestern part of india 5052.
The raw data were analyzed based on the agestage, twosex life table. The new records extend westward by approximately 500 km the known geographic distribution of the species, being the first report of the species in the northwestern region of the country. Reproductive organ of males was dissected to determine the testis in the pupal stage, 3dayold flies and 7dayold flies and observed under scanning electron microscopy sem and transmission electron. Molecular analysis of complex biological structures and processes increasingly requires sensitive methods for protein sequencing. Adham,hsinchib, a department of entomology, faculty of science, cairo university, giza, egypt b laboratory of theoretical ecology, department of entomology, national chung hsing university, taichung, taiwan received25february2004.
Chrysomya megacephala is geographically and ecologically ubiquitous and highly mobile, active whenever weather and climate allow, and unspecialized in its general biology, so that it is unlikely to provide evidence of relocation from the first three sources listed. Calliphoridae from royapuram fishing harbour, chennai, tamil nadu, india. The genus chrysomya contains a number of species including chrysomya rufifacies and chrysomya megacephala. Alimentary canal of the blow fly chrysomya megacephala f. Oct 29, 2015 stable reference genes are essential for accurate normalization in gene expression studies with reverse transcription quantitative polymerase chain reaction qpcr. As in other insects, olfaction of this species plays an important role in host location and is presumably mediated via transmembrane. The term old world blow fly is a derivative of both the associated family, calliphoridae blow flies, and the belief that the genus chrysomya originated in asia. Comparing species composition of passive trapping of adult. Chrysomya megacephala, the most forensically important blowfly species in malaysia lee et al.
The daily flight activity, seasonal abundance related to abiotic factors temperature, relative humidity and rainfall and reproductive potential of this species was. Omar, 2007 accelerating chrysomya megacephala maggot growth for forensic entomology cases. Methodology fifty males and fifty females of the first phenotype adult c. Chrysomya megacephala, more commonly known as the oriental latrine fly, is a member of the family calliphoridae blowflies. Apr 20, 20 esser jr 1991 biology of chrysomya megacephala diptera. A synanthropic fly, chrysomya megacephala, is a well known medical vector and forensic indicator. The blow fly chrysomya megacephala fabricius, 1784, commonly called. Ecology and behavior laboratory colonization of the blow flies, chrysomya megacephala diptera. Nov 25, 2017 study shows just how dirty houseflies are. Review of its biology, pest status, and spread around the.
Worldwide the blowfly family includes over species and 150 genera rognes 1991. Mohd iswadi ismail, and khairul osman, and ong, hua king and nurhaslina hassan, and ezlan elias, and kaswandi md. Esser jr 1991 biology of chrysomya megacephala diptera. The latter species has become established in the neotropics and appears to outcompete the former species. Jul 01, 2008 for adult dry weight, both species had the lightest weight at a density of 320 larvae60 g and significantly differed from those at other density conditions p 0. Ambia, and ahmad rohi ghazali, and jamaludin mohamed, and baharuddin hj. Chrysomya megacephala fabricius, 1794 references edit ramaraj, p. Named to honor the italian entomologist mario bezzi, this fly is widely distributed in asia, tropical africa, india, and papua new guinea. Larval density, temperature and biological aspects of chrysomya megacephala diptera. Sculpture on the minor worker is restricted to the middle and posterior portions. Pdf life table of chrysomya megacephala fabricius diptera. Detailed information on the biology and reproductive. Review of its biology, pest status, and spread around the world.
Calliphoridae using light microscopy and electron microscopy. Larval density, temperature and biological aspects of chrysomya megacephaladiptera. Chrysomya megacephala biodiversidad costa granadina y. This is the most common member of the genus in new guinea and in at least a large part of its very extensive range. Larvae of this species have been reported in association with human corpses in several cases 912. Reproductive organ of blow fly, chrysomya megacephala. The adult can be identified as metallic green or blue with a yellow face and the larvae are smooth, lacking any obvious body processes except on the last segment. Back to diptera index page back to arthropods of orange county, california back to natural history of orange county. Jul 23, 2014 nonbiting blow fly chrysomya megacephala is commonly found in dead bodies and is used in forensic investigations to determine the time of death, referred to as the post mortem interval. The larvae of this fly species develop generally in carrion or on dead and decomposed tissues 4 chrysomya megacephala fabricius also. Chrysomya megacephala is a forensically important blow fly distributed in many parts of world and is available throughout the year in northwestern part of india 4, 7, 8. Collection of insects at the scene is one of the most important aspects of forensic entomology and proper collection is one of the biggest challenges for any investigator. Hairy maggot blow fly, chrysomya rufifacies macquart 2 carrion to search of a pupation site.
Chrysomya bezziana, also known as the old world screwworm fly or screwworm, is an obligate parasite of mammals. Introduction the family calliphoridae has nonmetallic and metallic flies ambrose 2007. Hairy maggot blow fly, chrysomya rufifacies macquart. In this work, we evaluated the ovicidal activity and the deleterious effects of cashew anacardium occidentale nut shell oil and its fractions on the development of musca domestica and chrysomya megacephala, important vectors of several diseases. Chrysomya megacephala also exhibited a trni duplication, as reported either side of the dloop or large noncoding region in chrysomya putoria junqueira et al. Calliphoridae cannot only act as a mechanical vector of various pathogens, but also infest man and animals causing human health problems and economic losses in the livestock and fish industries. Report on the occurrence of synanthropic derived form of.
Pupation usually occurs within the first inch of topsoil or under leaf litter, rocks, or fallen limbs. Molecular characterization of the carbon dioxide receptor. Reproductive organ of males was dissected to determine the testis in the pupal stage, 3dayold flies and 7dayold flies and observed under scanning electron microscopy sem and transmission. Morphological comparison of the third instar of both species revealed different characteristics e. Unfortunately, previous studies did not look at the stability of reference genes used in c. During this time, the larval skin shrinks and hardens to form the puparium which is dark brown. The oriental latrine fly, chrysomya megacephala calliphoridae is of medical importance and distributed worldwide. Chrysomya albiceps is considered conspecific with chrysomya rufifacies by some authorities. In this study, chrysomya megacephala fabricius, 1794 was reared in two study. Chrysomya bezziana is distributed widely in southeast asia, new guinea, and africa. Report on the occurrence of synanthropic derived form of chrysomya megacephala diptera.
The daily flight activity, seasonal abundance related to abiotic factors temperature, relative humidity and rainfall and reproductive potential of this species was investigated. It is a warmweather fly with a greenishblue metallic boxlike body. Pdf larval density, temperature and biological aspects of. Larvae of this species have been reported in association with human corpses in several cases 5359. Calliphoridae and reduction of losses caused to the salteddried fish industry in southeast asia j. Morphology and developmental rate of blowflies chrysomya. The present study updates the distribution of chrysomya megacephala for argentina. Larvae of the latter can prey on other maggots, including that of their own species as facultative food. Chrysomya is an old world blow fly genus of the family calliphoridae. Chrysomya bezziana an overview sciencedirect topics. Adults strongly attracted to exposed foods, especially fish, meat and anything sweet, and to human excreta hence the name, the latrine fly. The minor workers are small, yellow to brown, small propodeal spines, and no antennal scrobes.
Originalarticle life table of chrysomya megacephala fabriciusdiptera. Accelerating chrysomya megacephala maggot growth for. Media in category chrysomya megacephala the following 30 files are in this category, out of 30 total. Ovicidal and deleterious effects of cashew anacardium.
Calliphoridae in the new world article pdf available in journal of medical entomology 211. Chrysomya megacephala fabricius is a prevalent and synanthropic blowfly which has two sides, for being a pathogenic vector, an efficient pollinator, a promising resource of proteins, lipids, chitosan, biofuel et al. Chrysomya megacephala is of particular interest for its. Chrysomya megacephala fabricius, the latrine fly is widespread throughout india and south east asia with many recent introductions in many parts of the world. Fecundity, body size and population dynamics of chrysomya. Callipho ridae has reached the continental united states. Mitochondrial genomes of the sheep blowfly, lucilia sericata. During my master study worked on the dissertation topic effects of modafinil on the life cycle of chrysomya megacephala.
Geographically, the distribution of this species is quite broad, extending from the oriental, australasian, and oceania regions to africa, south america, and central america braack 1991, kurahashi and magpayo 2000. Effect of dietary fat levels on the size and development. The term old world blow fly is a derivative of both the associated family, calliphoridae blow flies, and the belief that the genus chrysomya originated in asia and migrated to north america only relatively recently. Larval density, temperature and biological aspects of. Introduction chrysomya albiceps wiedemann, 1819 is a blowfly originary from the southern palaeartic region, north africa eastwards to northwest india, throughout africa, including afrotropical islands of aldabra, cape verde, madagascar, mauritius, reunion, rodriguez, st. Regarding total fecundity tf and net fecundity nf, the greatest values obtained in this study were found at density 100 replicate, followed by. Understanding its bionomics is essential for both designing effective fly control programs and its use in forensic investigations. The two species have a similar biology and the morphological differences are slight prostigmatic bristle present in c. Chrysomya megacephala and chrysomya rufifacies are two predominant necrophagous species in taiwan. The blowfly chrysomya megacephala fabricius diptera.
Development of immature stages was estimated on the basis of. It is a widely distributed carrionbreeding fly that commonly shifts from being a scavenger in. The life history of the oriental latrine fly, chrysomya megacephala fabricius, was studied at 26 c in the. Studies on biology of the blowfly chrysomya megacephala. Femtomole sequencing of proteins from polyacrylamide gels by. Survival percent of adults of males and females of c. Chrysomya megacephala propagates diseases like diarrhoea and parasite infestations 122,123. Jul, 2018 chrysomya megacephala is a blow fly species of medical and forensic importance worldwide. Biology, blowfly, chrysomya megacephala, fluctuating temperatures, gorakhpur condition. Demographic aspects of chrysomya megacephala diptera, calliphoridae adults brazilian archives of biology and technology 459 were pregnant within 10 days following their emergence. Chrysomya megacephala fabricius musca megacephala fabricius, 1784, syst.
The voucher specimens were deposited in the collection of the miguel lillo foundation institute institutofundacion miguel lilloimla. Bionomical parameters such as body size, development time, mortality, and sex ratio were observed. Calliphoridae, an important forensic blowfly species in china. The identification, geographical distribution and biology of the species are. Effect of dietary fat levels on the size and development of. Jan 23, 2015 chrysomya megacephala fabricius is a prevalent and synanthropic blowfly which has two sides, for being a pathogenic vector, an efficient pollinator, a promising resource of proteins, lipids, chitosan, biofuel et al. Pdf larval density, temperature and biological aspects. Chrysomya megacephala, more commonly known as the oriental latrine fly, is a member of the. The most important species are cochliomyia macellaria fig. The sample flies procured from the exposed beef liver were identified as lucilia sericata and chrysomya megacephala up to the species level with the help of relevant literature. Pdf the life history of the oriental latrine fly, chrysomya megacephala fabricius, was studied at 26 c in the laboratory.
Specieslevel identification of the blowfly chrysomya. Deadbodyfeeding larvae useful in forensic investigations. The raw data were the raw data were analyzed based on the agestage, twosex life table, in order to take both sexes and the variable developmental rate among individuals and. The life history of the oriental latrine fly, chrysomya megacephala fabricius, was studied at 26 c in the laboratory. Firstinstar larvae emerge firstinstar larvae emerge in 1. Electrospray mass spectrometry1 has been applied to the high. Calliphoridae and reduction of losses caused to the salteddried fish industry in southeast asia volume 81. Jun 01, 2011 chrysomya megacephala is reported by the first time to tucuman province, northwestern argentina. Based on the conditions of slit on posterior spiracle which could be used to discern larval instars barroscordeiro and pujolluz 2010, c.
Mitochondrial genomes of the sheep blowfly, lucilia. Bionomics of the oriental latrine fly chrysomya megacephala. Medium pheidole megacephala is a common species in the pacific with two waist segments, 12segmented antennae, 3segmented antennal clubs, propodeal spines, long thin pilosity, and a bimorphic worker caste. Pdf chrysomya megacephala fabricius, 1794 occurs on every continent and is closely.
Moreover olfactory proteins are crucial component to function in related processes. Calliphoridae and reduction of losses caused to the salteddried fish industry in southeast asia. Nonbiting blow fly chrysomya megacephala is commonly found in dead bodies and is used in forensic investigations to determine the time of death, referred to as the post mortem interval. Obligate parasitic flies require a host to complete their development. Femtomole sequencing of proteins from polyacrylamide gels. Life table of chrysomya megacephala fabriciusdiptera. Chrysomya megacephala fabricius is a wellknown blow fly species of medical importance worldwide. Dead body feeding larvae useful in forensic investigations. Reference gene stability of a synanthropic fly, chrysomya. The blowfly chrysomya megacephala, or oriental latrine fly, is the most common humanassociated fly of the oriental and australasian regions. The larval morphology and developmental rate of chrysomya megacephala f. Larvae feed as scavengers, and not normally in diseased tissue of mammals in contrast to the very similar sheep blowfly, chrysomya rufifacies. The fly infests corpses soon after death, making it important to forensic science.
This work presents the ultrastructure of testis of the medically important blow fly, chrysomya megacephala fabricius diptera. Adult flies are highly mobile and ubiquitous at scenes, yet their link to the body and the time of colonization toc and postmortem interval pmi estimates is not well established. Molecular characterization of the carbon dioxide receptor in. Pdf the uses of chrysomya megacephala fabricius, 1794. Like the new world screwworm, this species is attracted to open wounds as well as to moist body openings. Calliphoridae has reached the continental united states. Eight other calliphorid species were collected in the same locations. The developmental transcriptome of the synanthropic fly. The uses of chrysomya megacephala fabricius, 1794 diptera.
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